Sensibilidad in vitro de Cándida albicans a diversos antifúngicos

Abstract

We have studied the in vitro sensitivity of 8 strains of Candida albicans for 6 antifungal agents; 4 of the strains come from cow's milk and the other 4 from human vaginal exudate.

Minimal inhibitory concentrations (MICs) were obtained by the dilution method using Sabouraud dextrose broth as culture medium. Later on, the minimal fungicidal concentrations (MFCs) was established after subculture in Sabouraud dextrose agar.

The mean MICs and MFCs values for all strains were: trichomycin (0,019 and 0,34 pg/ml), amphotericin B (0,48 and 1,07 pg/ml), nystatin (1,56 and 3,71 ILg/ml), 5-fluorocytosine (10,94 and > 143,7 pg/ml), clotrimazole (14,84 and 81,25 ILg/ml) and miconazole (46,87 and 50 ILg/ml).

Zones of inhibition were established with the diffusion disk method, using Sabouraud dextrose agar as culture medium, and in the case of 5fluorocytosine it was also used the Yeast nitrogen base agar with L-asparagine and dextrose. Disks loaded with l, 15, 30 and 45 pg of antifungal agent were• used in all cases. The disks containing I Pg did not produce any significant zone of inhibition when the Sabouraud medium was used. The greates zones of inhibition corresponded to 5-fluorocytosine, followed by nystatin, trichomycin, clotrimazole, miconazole and much smaller zones of inhibition were found in the case of amphotericin B.